Autotransplantation is an excellent option for the treatment of missing teeth. However, the ideal clinical situation for this procedure is not always present. For example, the recipient site may be smaller than the donor tooth or there may be no suitable healthy donor tooth. Therefore, cryopreserved teeth stored in a teeth bank can be used in future orthodontic treatments. In this experiment, a novel freezing program with a slightly unidirectional magnetic field was used for the cryopreservation of orthodontic-extracted teeth. When supercooling tooth tissue under this magnetic field the electron spin effect of molecules allows the structure to vitrify instead of crystallize, which reduces cell damage during freezing. Preliminary results demonstrated that over 90% of periodontal ligament cells survived after cryopreservation using this method. In addition, the loss of alkaline phosphatase activity of PDL cells was lower than 10% compared to the control group. Finally, autotransplanted teeth cryopreserved in this method showed a 95% clinical survival rate.