Objective: The purpose of this research was to investigate in vitro the biological effects of orthodontic NiTi archwires on human oral squamous cell carcinoma.
Methods: A human oral squamous cell carcinoma line (OSCC-CR035–UFRJ,Brazil) was seeded in two plates of 12 wells of 2cm2 (TPP, Switzerland) and transferred to a 1.33 X 105 cell/well concentration. In sequence, the dishes were incubated for 24 hours, in 37°C and 70% humidity, in DMEM cell–penicillin/ streptomycin - 10 μg/ml (Gibco, USA). After 24 hours, wire segments of 1.5 cm were added to 1.5 ml of new culture medium - Dish 1 – nine wells with 0.012” NiTi wire (Morelli, Brazil)and three control (culture medium only)and Dish 2 - nine wells with 0.020” NiTi wire (Morelli, Brazil)and three control (culture medium only). The dishes were incubated again for more 48 hours, 37°C, 5%CO2 and 70% humidity. Cell viability was analyzed in a crystal violet assay.
Results: Statistical analysis revealed an absence of significative differences between the control, 0.012” and 0.020” NiTi wire groups (p>0,05).
Conclusion: In a 48 hour exposure observation period, 0,012” and 0.020” orthodontic archwires did not demonstrate any alteration in OSCC cells viability.